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PART I MOLECULAR BIOLOGY An Introduction to Molecular Biotechnology Genetic Material DNA Replication and Repair Gene Concept Transcription or Gene Expression Translation PART II GENETICS Regulation of Gene Expression Mendel’s Laws Gene Interaction Linkage and Crossing Over Mutations Bacterial Recombination Transposons Chloroplast and Mitochondrial Genome Organization PART III GENETIC ENGINEERING Gene Cloning Enzymes Used in Genetic Engineering Bacterial Vectors Blotting Techniques Generation of Clones DNA Libraries Polymerase Chain Reaction DNA Synthesis by Chemical Method Restriction Fragment Length Polymorphism Gene Transfer Methods Application of Recombinant Technology